| L-Selectin

L-selectin (SELL), also known as CD62L, is a cell adhesion molecule found on leukocytes and preimplantation embryos. It belongs to the selectin family of proteins and recognizes siliconed carbohydrate moieties. It is cleaved by ADAM17.L-selectin acts as a “homing receptor” for lymphocytes that enter secondary lymphoid tissues through high endothelial venules.Ligands present on endothelial cells will bind to lymphocytes expressing L-selectin, slowing lymphocyte trafficking in the bloodstream and facilitating entry into the secondary lymphoid organs at that point.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	10	5	2.5	1.25	0.62	0.31	0.15	0
OD	2.05	1.12	0.57	0.35	0.25	0.2	0.18	0.08
Corrected OD	1.97	1.04	0.49	0.27	0.17	0.12	0.1	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Rat L-Selectin spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-103	97
Plasma(n=8)	92-105	102
Cell Culture Supernates(n=8)	96-108	105

 

| Linearity

Samples were spiked with high concentrations of Rat L-Selectin and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-97	90-110
	Average (%)	91	93	96
1 : 4	Range (%)	87-103	87-108	105-115
	Average (%)	92	98	108